Considerable modulation of the hepatic proteome induced by exposure to reduced temperature in Xenopus laevis [Investigation Article]
- Kazumichi Nagasawaone,
- Yuta Tanizaki1,two,
- Takehito Okui1,
- Atsuko Watarai3,
- Shinobu Uedathree and
- Takashi Katoone,4,*
oneDepartment of Integrative Bioscience and Biomedical Engineering, Graduate School of Superior Science and Engineering, Heart
for Sophisticated Lifestyle and Healthcare Science, Waseda College, TWIns Constructing, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
twoJapan Society for the Marketing of Science (JSPS), Kojimachi Business Heart Developing, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan
3Institute for Innovation Design and style, Comprehensive Study Organization, Waseda University, TWIns Creating, two-two Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
fourOffice of Biology, Faculty of Education, Centre for Advanced Existence and Health-related Science, Waseda University, TWIns Constructing, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
- ↵*Writer for correspondence ( )
The African clawed frog, Xenopus laevis, is an ectothermic vertebrate that can endure at low environmental temperatures. To achieve perception into the molecular events
induced by minimal physique temperature, liver proteins ended up evaluated at the regular laboratory rearing temperature (22°C, management)
and a low environmental temperature (5°C, cold exposure). Using nano-flow liquid chromatography coupled with tandem mass spectrometry,
we recognized fifty eight proteins that differed in abundance. A subsequent Gene Ontology analysis exposed that the tyrosine and phenylalanine
catabolic processes ended up modulated by cold exposure, which resulted in decreases in hepatic tyrosine and phenylalanine, respectively.
Similarly, levels of pyruvate kinase and enolase, which are included in glycolysis and glycogen synthesis, had been also reduced,
whilst levels of glycogen phosphorylase, which participates in glycogenolysis, ended up elevated. Therefore, we calculated metabolites
in the respective pathways and found that ranges of hepatic glycogen and glucose ended up diminished. Even though the liver was beneath
oxidative stress since of iron accumulation triggered by hepatic erythrocyte destruction, the hepatic NADPH/NADP ratio was
not modified. As a result, glycogen is probably utilized mostly for NADPH source relatively than for strength or glucose production. In
conclusion, X. laevis responds to minimal entire body temperature by modulating its hepatic proteome, which outcomes in altered carbohydrate metabolic rate.
- Gained July 7, 2013.
- Recognized July 22, 2013.
- © 2013. Revealed by The Company of Biologists Ltd
This is an Open up Access article dispersed below the conditions of the Inventive Commons Attribution License (http://creativecommons.org/licenses/by/3.), which permits unrestricted use, distribution and reproduction in any medium provided that the original perform is properly