Useful coupling in between large-conductance potassium channels and Cav3.2 voltage-dependent calcium channels participates in prostate cancer cell expansion [Research Report]
- Florian Gackière*,
- Maritime Warnier*,
- Maria Katsogiannou,
- Sandra Derouiche,
- Philippe Delcourt,
- Etienne Dewailly,
- Christian Slomianny,
- Sandrine Humez,
- Natalia Prevarskaya,
- Morad Roudbaraki and
- Pascal Mariot‡
Laboratoire de Physiologie Cellulaire, INSERM U1003, Bâtiment SN3, Université Lille one, 59655 Villeneuve d’Ascq Cédex, France
- ↵‡Writer for correspondence ( )
↵* These authors contributed similarly to this function
It is strongly suspected that potassium (K+) channels are concerned in various aspects of prostate most cancers development, these kinds of as cell development. Nonetheless, the molecular mother nature
of individuals K+ channels implicated in prostate cancer mobile proliferation and the mechanisms by way of which they management proliferation are
still unknown. This examine makes use of pharmacological, biophysical and molecular ways to present that the primary voltage-dependent
K+ existing in prostate cancer LNCaP cells is carried by massive-conductance BK channels. Without a doubt, most of the voltage-dependent
present was inhibited by inhibitors of BK channels (paxillin and iberiotoxin) and by siRNA targeting BK channels. In addition,
we expose that BK channels represent the main K+ channel household concerned in setting the resting membrane prospective in LNCaP cells at all around −40 mV. This for that reason encourages
a constitutive calcium entry by way of T-sort Cav3.2 calcium channels. We show, using single-channel recording, confocal
imaging and co-immunoprecipitation methods, that the two channels type macromolecular complexes. Ultimately, making use of flow cytometry
mobile cycle measurements, cell survival assays and Ki67 immunofluorescent staining, we show that each BK and Cav3.two channels
participate in the proliferation of prostate most cancers cells.
Creator Contributions P.M. and M.R. supervised the examine. Patch-clamp research have been carried out by F.G. Proliferation studies have been performed by
M.W., F.G. and P.D. Co-immunoprecipitation studies had been executed by M.W., M.K., S.D., E.D. and C.S. Calcium measurements
ended up done by S.H. Data ended up analyzed by P.M., F.G. and M.W. The manuscript was written by P.M. and corrected by M.R.,
M.W., F.G. and N.P.
Competing passions The authors have no competing pursuits to declare.
- Obtained April 22, 2013.
- Accepted June 26, 2013.
- © 2013. Printed by The Organization of Biologists Ltd
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