Drosophila integrin adhesion complexes are vital for hemocyte migration in vivo [Investigation Article]
oneInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal
2CEDOC, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal
‡Current tackle: Carl Zeiss Microscopy GmbH, Königsallee 9-21, 37081 Göttingen, Germany
- ↵*Writer for correspondence ( )
Cell migration is an critical organic process which has been intensively studied in the earlier many years. Quite a few techniques,
mostly involving two-dimensional mobile tradition techniques, have contributed to dissecting the essential mechanisms fundamental
this process. Nonetheless, the development of 3-dimensional mobile lifestyle and in vivo programs has proven some distinctions with
what was formerly believed to be properly-proven mobile migration mechanisms, suggesting that two-dimensional mobile motility
would be a very poor predictor of in vivo conduct. Drosophila is a widely regarded product organism to review developmental and homeostatic procedures and has been broadly used to examine
cell migration. Below, we emphasis on the migration of tiny groups of pupal hemocytes that accumulate for the duration of larval phases in
dorsal patches. We show that integrins, and other acknowledged nascent adhesion-associated proteins this sort of as Rhea and Fermitin 1, are
essential for this method and that their depletion does not impact polarization in reaction to environmental cues. We also
current evidence for the value of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion
in hemocytes leads to a substantial improve of mobile pace with out influencing their reaction to a chemotactic cue. This is
the first report of a systematic investigation employing Drosophila melanogaster hemocytes to examine adhesion-associated proteins and their purpose in cell migration in vivo. Our data level to mechanisms of
mobile migration related to these described in 3-dimensional in vitro programs and other in vivo product organisms.
Writer contributions C.G.A.M., A.J. and S.P. conceived and made the experiments. C.G.A.M. performed the experiments and analyzed the info.
C.G.A.M., A.J. and S.P. wrote the paper.
Competing passions The authors have no competing interests to declare.
- Received February 22, 2013.
- Approved May possibly one, 2013.
- © 2013. Released by The Organization of Biologists Ltd
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