hsa-miR29b, a vital downstream concentrate on of non-canonical Wnt signaling, performs an anti-proliferative position in non-tiny cell lung cancer cells by way of targeting MDM2 expression [Research Report]
- Sreedevi Avasarala1,
- Michelle Van Scoykone,
- Jianbin Wang2,
- Marybeth Sechler1,
- Katherine Vandervest1,
- Christine Brzezinski1,
- Colin Weekes3,
- Michael G. Edwardsone,
- John Arcarolithree,
- Richard E. Davistwo,
- Rama Kamesh Bikkavilli1 and
- Robert A. Winnone,four,*
1Division of Pulmonary and Essential Care Sciences, University of Medication, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, United states
twoDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Colorado, Aurora, CO 80045, United states
threeDivision of Medical Oncology, Faculty of Drugs, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, Usa
4Veterans Affairs Medical Centre, Denver, CO 80220, Usa
- ↵*Writer for correspondence ( )
In non-modest cell lung cancer cell traces, activation of β-catenin impartial signaling, through Wnt7a/Frizzled9 signaling, prospects
to reversal of cellular transformation, diminished anchorage-impartial growth and induction of epithelial differentiation.
miRNA expression profiling on a human lung adenocarcinoma cell line (A549) identified hsa-miR29b as an important downstream
target of Wnt7a/Frizzled9 signaling. We show herein that hsa-miR29b expression is dropped in non-tiny cell lung cancer (NSCLC)
cell strains and stimulation of β-catenin independent signaling, through Wnt7a expression, in NSCLC cell traces final results in enhanced
expression of hsa-miR29b. Surprisingly, we also determine particular regulation of hsa-miR29b by Wnt7a but not by Wnt3, a ligand
for β-catenin-dependent signaling. Interestingly, knockdown of hsa-miR29b was adequate to abrogate the tumor suppressive effects
of Wnt7a/Frizzled9 signaling in NSCLC cells, suggesting that hsa-miR29b is an critical mediator of β-catenin independent
signaling. Last but not least, we display for the first time that hsa-miR29b performs an essential function as a tumor suppressor in lung most cancers
by focusing on murine double mutant 2 (MDM2), revealing novel nodes for Wnt7a/Frizzled9-mediated regulation of NSCLC mobile proliferation.
- Obtained February eighteen, 2013.
- Acknowledged April eight, 2013.
- © 2013. Revealed by The Firm of Biologists Ltd
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